Signalling

Part:BBa_K2996704:Design

Designed by: Yunqing Wang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-10-14)


target sequence upstream of promotor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
    Illegal NheI site found at 75
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 39
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

With the transcription start denoted +1, RNAP complex covers from -55 to +20. sgRNA that targets this region can be used for transcription activation.


Source

None.

References