Other
Part:BBa_K2996504:Design
Designed by: Shifeng Jiang Group: iGEM19_SJTU-BioX-Shanghai (2019-10-03)
Binding sites for dCas9 and zinc finger
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 24
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 24
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 24
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We devised the target DNA sequence of NdCas9 after carefully checking if there is any similar sequence amongst the plasmids.
Source
Synthesis