Composite

Part:BBa_K2996008:Design

Designed by: Yunqing Wang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-10-04)


pLuxR upstream of cas1/2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 258
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 979
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

The plasmid backbone containing luxR and pLuxR is donated from 2017 iGEM-Tsinghua. We constructed pLux-cas using gibson assembly.

References