Coding

Part:BBa_K2996000:Design

Designed by: Yunqing Wang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-10-02)


repL, replication initiator protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 377
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 308
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

cas1 is 918bp, cas2 is 285bp. And they are spaced by 1bp.


Source

The sequence is cloned from E.coli K12 genome into pRec plasmid by professor Wang's lab. They kindly donated this plasmid to us.

References