Composite

Part:BBa_K2994001:Design

Designed by: Qitong Luan   Group: iGEM19_Gaston_Day_School   (2019-10-06)


Temperature Sensitive GFP Production Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

J23100 is a strong constitutive promoter, and B0035 is strong RBS. The combination of them increases the efficiency for the production of gTEMP.


Source

Synthetic construct of a combination of Sirius and mT-Sapphire

References

Nakano, M. Arai, Y. Kotera, I. Okabe, K. Kamei, Y. Nagai, T. (2017). Genetically encoded ratiometric fluorescent thermometer with wide range and rapid response. PLOS ONE, 12(2), e0172344. https://doi.org/10.1371/journal.pone.0172344