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Part:BBa_K2992048:Design

Designed by: Yaseen Tengur   Group: iGEM19_Nottingham   (2019-10-01)


FAST reporter construct with Pj23106 and thl RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 231


Design Notes

FAST is originally derived from Halorhodospira halophile and has been codon optimised for Clostridium spp. [Street et al, 2019 DOI: 10.1128/AEM.00622-19].


Source

C. acetobutylicum, C. pasteurianum and E. coli DNA. FAST is originally derived from Halorhodospira halophile and has been codon optimised for Clostridium spp. [Street et al, 2019 DOI: 10.1128/AEM.00622-19].

References