Composite

Part:BBa_K2992036:Design

Designed by: Yaseen Tengur   Group: iGEM19_Nottingham   (2019-09-26)

Design considerations

Amplified unmodified from the genome sequences of the host organisms. The ctfA and ctfB genes from the iGEM 2015 entries M36581 BBa_ M36581 and M36582 BBa_ M36582 list the reverse complements of the coding sequences. In our entry we use the same sequences but in the correct 5’-3’ orientation. The RBS for ctfB is contained within the coding sequence for ctfA.