Coding
YFP

Part:BBa_K2984075:Design

Designed by: Dimitri Schumacher   Group: iGEM19_Humboldt_Berlin   (2019-10-19)


YFP - Yellow fluorescent protein for detection


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 202
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 202
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 202
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 202
    Illegal NgoMIV site found at 679
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was used to make our composite parts compatible for the iGEM registry. This part is not MoClo compatible because it does not have the MoClo overhangs!


Source

Ormo, M., Cubitt, A. B., Kallio, K., Gross, L. A., Tsien, R. Y., & Remington, S. J. (1996). Crystal structure of the Aequorea victoria green fluorescent protein. Science, 273(5280), 1392-1395.

References