Coding

Part:BBa_K2984038:Design

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-16)


L1c-Psad-CBA-Rbcs2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1106
    Illegal PstI site found at 1453
    Illegal PstI site found at 1826
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1106
    Illegal PstI site found at 1453
    Illegal PstI site found at 1826
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal BamHI site found at 1085
    Illegal XhoI site found at 1271
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1106
    Illegal PstI site found at 1453
    Illegal PstI site found at 1826
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1106
    Illegal PstI site found at 1453
    Illegal PstI site found at 1826
    Illegal NgoMIV site found at 1208
    Illegal NgoMIV site found at 2095
    Illegal AgeI site found at 2036
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Selection marker, codon optimized for transformation in C.reinhardtii and further cloning into Level 2 backbone with MoClo standard



Source

The construct consist of the four different Level 0 parts: Psad (BBa_K2984008), PETase (), ptxD (BBa_K2984013) and Rbcs2 (BBa_K2984018) which were cloned into the level 1 Backbone (BBa_K2984002) using the MoClo Standard.


References