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Coding
Part:BBa_K2984036:Design
Designed by: Dimitri Schumacher Group: iGEM19_Humboldt_Berlin (2019-10-16)
L1c-PsaD-PETase-YFP-RBCS2
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1915
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This construct was designed on a level 1 c backbone (BBa_K2984002). Together with L1a (BBa_K2984003) and L1b (BBa_K2984005), a level 2 construct can be built for multiple gene insertion. A B2-B2 linker was used to assemble this construct between PsaD and PETase.
Source
The construct was built out of 4 different level 0 plasmids using the MoClo standard (listed in the main description).