Coding

Part:BBa_K2984027:Design

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-16)


L1c-Psad-ARS-YFP-Rbcs2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1110
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1110
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1110
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1110
    Illegal NgoMIV site found at 1587
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Plasmid could be used for second MoClo reaction for cloning into level 2 plasmid


Source

The construct consist of the four different Level 0 parts Psad (BBa_K2984008),ARS (BBa_K2984000), YFP (BBa_K2984017) and Rbcs2 (BBa_K2984018) which were cloned into the level 1 Backbone (BBa_K2984002) using the MoClo standard

References