Coding

Part:BBa_K2984019:Design

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-15)


L1c-Psad-YFP-RbcS2, Expression of YFP through light-inducible promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1046
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1046
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1046
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1046
    Illegal NgoMIV site found at 1523
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codonoptimization of part, Use of promoter, terminator respectively that originates from targeted locus.


Source

Promoter ist part of 5'UTR of photosystemI reaction center subunit II-1. The terminator originates form 3'UTR Ribulose-1,5-bisphosphat-carboxylase.

References