Part:BBa_K2984018

RbcS2 - Terminator B5-C1

This part is a part of the Chlamy-HUB-Collection. To terminate the transcription of a gene a signal on the DNA is required. The nucleotide sequence mediating this signal to the RNA-polymerase is called terminator. In 1986 a Chlamydomonas reinhardtii native gene for the small subunits of the ribulose bisphosphate carboxylase/oxygenase (Rubisco) was characterized (Goldschmidt-Clermont and Rahire, 1968; Stevens et al., 1996).

This part was designed to be used with the MoClo standard and has B5-C1 overhangs.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 258
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization

Fig.1 - Cloning scheme of L0-RbcS2_B5-C1 into E. coli. A RbcS2 B5-C1 PCR product on agarose gel. B + C Successful transformation into E. coli. D + E Sequencing results of L0-RbcS2_B5-C1.

References

1. Goldschmidt-Clermont, M., & Rahire, M. (1986). Sequence, evolution and differential expression of the two genes encoding variant small subunits of ribulose bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Journal of molecular biology, 191(3), 421-432.
2. Stevens, D. R., Purton, S., & Rochaix, J.-D. (1996). The bacterial phleomycin resistance geneble as a dominant selectable marker inChlamydomonas. Molecular and General Genetics MGG, 251(1), 23–30. https://doi.org/10.1007/BF02174340