Tag
3xHA B5-B5

Part:BBa_K2984015:Design

Designed by: Sophia Schiebler   Group: iGEM19_Humboldt_Berlin   (2019-10-14)


3x HA-Tag for protein detection, isolation, purification B5-B5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The amino acid sequence was also translated to base triplets in a codon optimized way for C. reinhardtii.


Source

Silflow, C. D., LaVoie, M., Tam, L. W., Tousey, S., Sanders, M., Wu, W., . . . Lefebvre, P. A. (2001). The Vfl1 Protein in Chlamydomonas localizes in a rotationally asymmetric pattern at the distal ends of the basal bodies. The Journal of cell biology, 153(1), 63-74. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/11285274

References

Wilson, I. A., Niman, H. L., Houghten, R. A., Cherenson, A. R., Connolly, M. L., & Lerner, R. A. (1984). The structure of an antigenic determinant in a protein. Cell, 37(3), 767-778. Retrieved from https://doi.org/10.1016/0092-8674(84)90412-4. doi:10.1016/0092-8674(84)90412-4 Silflow, C. D., LaVoie, M., Tam, L. W., Tousey, S., Sanders, M., Wu, W., . . . Lefebvre, P. A. (2001). The Vfl1 Protein in Chlamydomonas localizes in a rotationally asymmetric pattern at the distal ends of the basal bodies. The Journal of cell biology, 153(1), 63-74. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/11285274