Part:BBa_K2984005:Design

Level 1 b backbone with GoldenGate Fusionsite

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BamHI site found at 729
  Illegal XhoI site found at 721
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal AgeI site found at 1535
  Illegal AgeI site found at 1647
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 1823
  Illegal BsaI.rc site found at 752
  

Design Notes

The codon usage optimization had to be adjusted to Chlamydomonas reinhardtii.

Source

Inspired by the backbone “pICH47732” created by Sylvestre Marillonnet (Weber et al., 2011)[1]

References

Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S (2011) A Modular Cloning System for Standardized Assembly of Multigene Constructs. PLOS ONE 6(2): e16765. https://doi.org/10.1371/journal.pone.0016765