Coding
GLE B2-B2
Part:BBa_K2984001
Designed by: Sophia Schiebler Group: iGEM19_Humboldt_Berlin (2019-10-03)
3'UTR, Secretion signal of gametolysin GLE B2-B2
This vector is a part of the Chlamy-HUB-Collection.It was shown that the putative signal sequence of the metalloprotease gametolysin (GLE) from C. reinhardtii can induce secretion (Ramos-Martinez, Fimognari, & Sakuragi, 2017). The amino acid sequence of it is MSLATRRFGAAAALLVAACVLCTAPAWA.
This part was designed to be used with the MoClo standard and has B2-B2 overhangs.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
References
- Ramos‐Martinez, E. M., Fimognari, L., & Sakuragi, Y. (2017). High‐yield secretion of recombinant proteins from the microalga Chlamydomonas reinhardtii. Plant biotechnology journal, 15(9), 1214-1224.
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Categories
Parameters
//awards/basic_part
None |