Reporter

Part:BBa_K2983060:Design

Designed by: Tom Zaplana   Group: iGEM19_Evry_Paris-Saclay   (2019-10-12)


RedStar2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Derived from the Addgene plasmid #120779.

The 3 EcoRI sites (GAATTC) were changed to GAgTTC.

The 3 BbsI sites (GAAGAC) were changed to GAgGAC or GAAaAC.

The BsmBI site (CGTCTC) was changed to CaTCTC.

All these mutations are synonymous.


Source

DNA synthesis