Composite

Part:BBa_K2981013:Design

Designed by: Junjie Zhu   Group: iGEM19_NWU-China   (2019-10-10)


For phenylalanine-sensitive measurement pathways


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1822
    Illegal BglII site found at 2164
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1628
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 800


Design Notes

NO


Source

DNA synthesis

References

[1] Chieh Lin, Yung-Cheng Jair, Yi-Chieh Chou, etc. Transcription factor-based biosensor for detection of phenylalanine and tyrosine in urine for diagnosis of phenylketonuria[J]. Analytica Chimica Acta,2018,1041(24):108-113.
[2] Pittard, J., Camakaris, H. Yang, J. (2005), The TyrR regulon. Molecular Microbiology, 55: 16-26.
[3] H.A. Harper, Review of Physiological Chemistry, (fourteenth ed.), Lange Medical Publications, Los Altos, CA (1973)