Part:BBa_K2981003
PtyrP, a phenylalanine-sensitive promoter regulated by TyrR protein
The tyrP promoter ,which codes for a component of the tyrosine-specific transport system, has been localized on the Escherichia coli K-12 chromosome at min 42. The tyrR gene product complexed with phenylalanine promotes transcription of tyrP.
Literature Characterization by AFCM-Egypt 2022
TyrP prompter activity is mediated by the presence of TyrR protein, In this experiment,the change of TyrP promoter activity was measured according to the nature of different DNA templates.The TyrP promoter was significantly expanded in the supercoiled form in the presence of RNAP, indicating that RNAP forms a more stable complex with the supercoiled TyrP promoter. However, only mild opening was identified at the upstream binding site's 10 areas when linear template was present.
Characterization by mathematical modeling by AFCM-Egypt 2022
We are using mathematical modeling to detect the increased level of phenylalanine (phe) in phenylketonuria patients in our diagnostic platform. It depends on a whole cell-based biosensor through a cascade of reactions to finally end by formation of β-galactosidase that turns the color into blue once bound to its substrate (X-gal) as mentioned in figure (4) and graph (1).
Figure (4) represents the cascade of reactions in whole cell-based biosensor model.
Graph(1) illustrates a direct relation between biomarker and beta-galactosidase ,so as the biomarker increases, the released amount of beta-galactosidase increases till it reaches constant value after about 30 time units. Therefore, the maximum amount of the biomarker releases the maximum amount of beta-galactosidase.
Experimental Characterization by AFCM-Egypt 2022 team
This figure shows an experimental characterization of this part as it's validated through gel electrophoresis as it is in lane 6 (the last one). The running part (ordered from IDT) included T7P - TyrR RBS - TyrR - TyrPromoter.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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