Coding
PhASE #2

Part:BBa_K2980014:Design

Designed by: Hongrui Liu   Group: iGEM19_Tsinghua   (2019-10-16)


(SIM-SUMO)3-SUMO3-mEGFP-Cry2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 393
    Illegal BglII site found at 852
    Illegal BamHI site found at 1331
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 277
    Illegal AgeI site found at 1006
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 629
    Illegal BsaI.rc site found at 38
    Illegal SapI.rc site found at 146


Design Notes

We should not fuse any protein to the N-terminus of Cry2, in case of any effect of its function.


Source

The Cry2 is from arabidopsis.

References