Coding
Part:BBa_K2980012:Design
Designed by: Zikang Huang Group: iGEM19_Tsinghua (2019-10-16)
Cry2-mCherry-Rluc
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 393
Illegal BglII site found at 852
Illegal BamHI site found at 1331 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 277
Illegal AgeI site found at 1006 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 629
Illegal BsaI.rc site found at 38
Illegal BsaI.rc site found at 2871
Illegal SapI.rc site found at 146
Design Notes
N terminal of cry2 is used to recognize CIB1, so that any attached protein should be fused at its C terminal. The linker between Rluc and mcherry should have enough amino acids.
Source
Rluc part is from Part:BBa_K1722005. The rest part is from Part:BBa K2980006.