Coding

Part:BBa_K2980012:Design

Designed by: Zikang Huang   Group: iGEM19_Tsinghua   (2019-10-16)


Cry2-mCherry-Rluc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 393
    Illegal BglII site found at 852
    Illegal BamHI site found at 1331
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 277
    Illegal AgeI site found at 1006
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 629
    Illegal BsaI.rc site found at 38
    Illegal BsaI.rc site found at 2871
    Illegal SapI.rc site found at 146


Design Notes

N terminal of cry2 is used to recognize CIB1, so that any attached protein should be fused at its C terminal. The linker between Rluc and mcherry should have enough amino acids.


Source

Rluc part is from Part:BBa_K1722005. The rest part is from Part:BBa K2980006.

References