Part:BBa_K2980012
Cry2-mCherry-Rluc
Rluc (Part:BBa_K1722005) was linked to the C terminal of Cry2-mCherry (Part:BBa_K2980006) and co-transformed with CIB1-GCN(4)-mEGFP-FUSLCD （Part:BBa_K2980009) to make it possible for Rluc to be recruited into phase. Then, as a control, we eliminated every component in both plasmid that may cause phase separation. Also, in order not to disturb the enzymatic activity, Rluc in both experimental group and control group were linked to mCherry. In detail, control group contained CIB1-mEGFP and mCherry-Rluc. We expected that after cultured in light, those two group would exhibit different enzymatic activity. Because phase could enrich both enzyme and substrate, bacteria in experimental group would perform higher catalytic activity compared to control group.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 393
Illegal BglII site found at 852
Illegal BamHI site found at 1331 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 277
Illegal AgeI site found at 1006 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 629
Illegal BsaI.rc site found at 38
Illegal BsaI.rc site found at 2871
Illegal SapI.rc site found at 146
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