Coding
Endolysin

Part:BBa_K2978000:Design

Designed by: Jose Pablo Delgado Navarro   Group: iGEM19_Costa_Rica   (2019-10-09)


Causes cell lysis of the pathogen Clostridium difficile


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Needs to be expressed in a chassis with T7 polymerase.

Source

Synthesized de novo with codon optimization for E.coli. The sequence was obtained from Wang et al. (2015) work.

References

Wang, Q., Euler, C. W., Delaune, A. & Fischetti, V. A. (2015). Using a Novel Lysin To Help Control Clostridium difficile Infections. Antimicrobial Agents and Chemotherapy, 59(12), 7447–7457. doi:10.1128/aac.01357-15