Part:BBa_K2973015

Hepatitis B Virus fragment (281-560bp)

Hepatitis B is a DNA virus that attacks the liver and can cause both acute and chronic diseases. This part represents a small fragment of the virus's genome. It is a small DNA fragment(280 bp), that does not encode for any functional protein or toxin. It's a small part of a sequence that encodes for a DNA polymerase of the virus and in alternative reading frames it encodes for envelope proteins of the virus, or for a specific surface antigen.

Usage and Biology

This part serves as a DNA template for amplification reactions (biomarker). In our project, we successfully amplified a specific region of the part using PCR and Recombinase Polymerase Amplification (RPA).

PCR Reaction:

RPA reaction after cleanup of the amplified product:

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 210
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]