Composite
Part:BBa_K2973000:Design
Designed by: Nick Delkis Group: iGEM19_Thessaly (2019-08-17)
T7-RBS-β-Lactamase-No Signal peptide
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 851
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Beta-lactamase needs to be truncated in its N-terminal end in order to work properly. Therefore, we deleted the signal peptide from its sequence in order to do our in vitro experiments.
Source
NCBI Reference Sequence: WP_000027057.1
References
Qureshi, Sohail A. “β-Lactamase: an Ideal Reporter System for Monitoring Gene Expression in Live Eukaryotic Cells.” BioTechniques, vol. 42, no. 1, 2007, pp. 91–96., doi:10.2144/000112292.
Boehle, Katherine E., et al. “Paper-Based Enzyme Competition Assay for Detecting Falsified β-Lactam Antibiotics.” ACS Sensors, vol. 3, no. 7, 2018, pp. 1299–1307., doi:10.1021/acssensors.8b00163.