Composite
Part:BBa_K2967028:Design
Designed by: Shuang Wu Group: iGEM19_NEU_CHINA (2019-10-06)
Expressing vector for YebF-Myrosinase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 689
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 918
Illegal BsaI site found at 1947
Design Notes
We need myrosinase to mediate the conversion of glucosinolate to sulforaphane. So we added a YebF secretory tag to secret this protein.
Source
The myrosinase gene comes from cruciferous vegetable.