Regulatory
Part:BBa_K2967021
Designed by: Lijun Bao Group: iGEM19_NEU_CHINA (2019-10-02)
PytfE promoter, responsive to nitrate, nitrite and nitric oxide
The promoter PytfE is sensitive to nitrate and nitrite. When nitrate and nitrite enter E. coli, they are converted to nitric oxide. Nitric oxide binds to the repressor protein NsrR, which inactivates PytfE to inhibit transcription of downstream genes. Then the promoter PytfE will be activated to express the luciferase.
reference
[1]Justino, M.C., et al., Escherichia coli YtfE is a di-iron protein with an important function in assembly of iron-sulphur clusters. FEMS Microbiol Lett, 2006. 257(2): p. 278-84.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |