Regulatory

Part:BBa_K2967021

Designed by: Lijun Bao   Group: iGEM19_NEU_CHINA   (2019-10-02)


PytfE promoter, responsive to nitrate, nitrite and nitric oxide

The promoter PytfE is sensitive to nitrate and nitrite. When nitrate and nitrite enter E. coli, they are converted to nitric oxide. Nitric oxide binds to the repressor protein NsrR, which inactivates PytfE to inhibit transcription of downstream genes. Then the promoter PytfE will be activated to express the luciferase.

reference

[1]Justino, M.C., et al., Escherichia coli YtfE is a di-iron protein with an important function in assembly of iron-sulphur clusters. FEMS Microbiol Lett, 2006. 257(2): p. 278-84.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None