Composite

Part:BBa_K2967019

Designed by: Lijun Bao   Group: iGEM19_NEU_CHINA   (2019-10-02)


The yeaR promoter will initiate the express of IL-10 when nitric oxide appear

In order to see whether the nitric oxide sensor will work when it comes to expressing a certain protein, we add the IL-10 downstream and demonstrated the western blotting. It has been proved that the expression level can be induced when adding nitric oxide.

800px-T--NEU_China--part--pyear-il10-1.png

Figure 1. Diagram of expressing vector in backbone pCDFDuet-1. Promoter yeaR is an nitric oxide inducible promoter. Gene hIL-10 is one of our target protein.

800px-T--NEU_China--part--pyear%2Bli10-2.png

Figure 2. Protein expressing of human il-10 gene which is transformed into BL21 strain and induced by nitric oxide. After induction of nitric oxide, the culture is incubated at 37℃ for 2 hours. Western blotting demonstrats the expression level of hIL-10. In order to equate the total number of cells in each lane, dilution is made to keep each sample at the same OD and sample volume.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
//collections/immune_regulation/devices
Parameters
None