Plasmid_Backbone

Part:BBa_K2962001:Design

Designed by: Handan KESIM   Group: iGEM19_Saint_Joseph   (2019-06-17)


PET26b


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 326
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 192
    Illegal NotI site found at 165
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 192
    Illegal BglII site found at 392
    Illegal BamHI site found at 198
    Illegal XhoI site found at 158
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 326
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 326
    Illegal NgoMIV site found at 231
    Illegal NgoMIV site found at 424
    Illegal NgoMIV site found at 2012
    Illegal NgoMIV site found at 2172
    Illegal NgoMIV site found at 5219
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 3092


Design Notes

We digested the vector with HindIII and NdeI enzymes to place laccase gene in the appropriate region.


Source

It has been ordered from a company called Novagen.

References