Coding

Part:BBa_K2959009:Design

Designed by: Alejandro Aguirre Hernndez   Group: iGEM19_Tec-Chihuahua   (2019-10-12)


Arabidopsis thaliana Profilin 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of AtPFN1 is succeeded by a 6x His-Tag to facilitate its purification by immobilized metal affinity chromatography.



Source

The full amino acid sequence was retrieved from Christensen et al. (1996).


References

1. Christensen, H. E. M., Ramachandran, S., Tan, C.-T., Surana, U., Dong, C.-H., & Chua, N.-H. (1996). Arabidopsis profilins are functionally similar to yeast profilins: identification of a vascular bundle-specific profilin and a pollen-specific profilin. The Plant Journal, 10(2), 269–279. doi:10.1046/j.1365-313x.1996.10020269.x