Coding

Part:BBa_K2959006:Design

Designed by: Carla Alejandra Pay�n Ram�rez   Group: iGEM19_Tec-Chihuahua   (2019-10-05)


Wheat antimicrobial peptide 1b from Triticum kiharae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 35


Design Notes

The sequence of WAMP1b is preceded by a 6x His-Tag to facilitate its purification by immobilized metal affinity chromatography.


Source

Amino acid sequence retrieved from Odintsova et al., (2009).

References

1. Odintsova, T. I., Vassilevski, A. A., Slavokhotova, A. A., Musolyamov, A. K., Finkina, E. I., Khadeeva, N. V., … Egorov, T. A. (2009). A novel antifungal hevein-type peptide fromTriticum kiharaeseeds with a unique 10-cysteine motif. FEBS Journal, 276(15), 4266–4275. doi: 10.1111/j.1742-4658.2009.07135.x