Regulatory

Part:BBa_K2957059

Designed by: Ethan Levy   Group: iGEM19_MIT   (2019-10-19)


CMV

Contents: CMV

What is it?: A basic part; it is a promoter and allows for constitutive expression of a gene.

What does it do?: This promoter expresses continually, and was put in our genetic circuits to continually produce the chemokines we were testing.

How to use it?: This part was used in Type IIS Cloning when we made our composite parts. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 92
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 92
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 92
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 92
  • 1000
    COMPATIBLE WITH RFC[1000]


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