Composite

Part:BBa_K2957038:Design

Designed by: Ethan Levy   Group: iGEM19_MIT   (2019-10-20)


hEF1a, EYFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1013
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1013
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1013
    Illegal AgeI site found at 81
    Illegal AgeI site found at 1019
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We needed to consider which cell type this plasmid would go into and the transfection efficiency was often dependent on promoter in the genetic circuit/construct.


Source

This part was from our mentor Shiva Razavi, who gave us the part when we were interested in potentially pursuing the HL60 cell to HEK cell communication synNotch system as a proof of concept of chemotaxis.

References