Composite
Part:BBa_K2957014:Design
Designed by: Melody Wu Group: iGEM19_MIT (2019-10-19)
hEF1a, CCL5
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 535
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 535
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 535
Illegal AgeI site found at 81
Illegal AgeI site found at 541 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used Modular Cloning Golden Gate Type II Assembly, so we had to consider how parts came together. We also used the natural secretion tag given in the part so we could use it to test chemokine secretion success by HEKs. We also had to consider which terminal the fluorescence/protein tag was added so as not to render the chemokine dysfunctional. See more on the MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT>.
Source
Parts of this part were ordered from IDT/Twist and then assembled with other parts (from iGEM/parts in our parts collection/from the Weiss lab) into this plasmid.