Composite

Part:BBa_K2957014

Designed by: Melody Wu   Group: iGEM19_MIT   (2019-10-19)


hEF1a, CCL5

Contents: hEF1a, CCL5, SynthpA, with Carb resistance

What is it?: A Composite part; it is a genetic circuit with hEF1a promoter and CCL5 gene allowing for constitutive CCL5 expression

What does it do?: It codes for the chemokine, CCL5, which is a chemoattractant for T cells. It was important for our project in particular because of its involvement in the immune system.

How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of CCL5. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 535
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 535
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 535
    Illegal AgeI site found at 81
    Illegal AgeI site found at 541
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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