Coding

Part:BBa_K2951007:Design

Designed by: YU-RU LIN   Group: iGEM19_CSMU_Taiwan   (2019-09-08)


Influenza A hemagglutinin with poly linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 291
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1.Codon optimization: We optimized the sequence's codon usage for E.coli.

2.RFC 10 compatible: We checked for restriction sites for EcoRI, XbaI, SpeI, PstI and NotI in order to make it RFC compatible.

3.Deletion of transmembrane domain: To improve the solubility of hemagglutinin, we predicted the transmembrane domain with TMHMM Server v. 2.0.

Source

The source of this part is from Influenza A virus (A/WSN/1933 TS61(H1N1)) segment 4, complete sequence, gene:20-1717 The sequence was obtained from NCBI(https://www.ncbi.nlm.nih.gov/nucleotide/CY010788.1?report=genbank&log$=nuclalign&blast_rank=2&RID=F439K4B5015)

References