![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K2950003
Designed by: Shuxin Xiao Group: iGEM19_BNDS_China (2019-10-16)
toluene dihydrodiol dehydrogenase(TodD) in P.putida
toluene dihydrodiol dehydrogenase(TodD) is identified in Pseudomonas putida, which catalyze the dehydrogenation process of of cis-hexadecylbenzene dihydrodiol to urushiol. TodD has a size of 30 kDa, we synthesized it via E.coli, and identified by SDS-PAGE electrophoresis. However, since the concentration of TodD was low, we construct a model on the expression of this particular protein, and tried to find the optimum.
![](https://2019.igem.org/wiki/images/4/44/T--BNDS_China--todD.jpg)
toluene dihydrodiol dehydrogenase(TodD) is identified in Pseudomonas putida, which catalyze the dehydrogenation process of of cis-hexadecylbenzene dihydrodiol to urushiol.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 381
Illegal NgoMIV site found at 469
Illegal AgeI site found at 736 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |