Coding

Part:BBa_K2947001:Design

Designed by: Shan Huang   Group: iGEM19_GDSYZX   (2019-10-13)


UGT33


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 624
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 34
    Illegal SapI site found at 460


Design Notes

We did codon optimization to the gene and deleted illegimate restriction.


Source

sequence from Dr.Jin Honglei's lab

References