![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K2940006:Design
Designed by: Longjiao Chen Group: iGEM19_Edinburgh_OG (2019-10-16)
Coding Sequence of super folding GFP with an N-terminal SpyCatcher
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 222
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 592
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A 2xGGGGS flexible linker was introduced between sfGFP and SpyCatcher to make sure the domains of two proteins will not be interfering with each other. 6XHis tag was also added at the N-terminus of the coding sequence for purification reasons.
Source
Super folding GFP was from iGE part BBa_K515005. The SpyCatcher was acquired by iGEM part BBa_K1650037