Coding
P20 with

Part:BBa_K2938008:Design

Designed by: Assaf Vital   Group: iGEM19_BGU_Israel   (2019-09-28)


P20 with His tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 264
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 264
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 264
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 264
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 264
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

His-tag fused to C- terminal P20 was optimized for E.Coli


Source

Gibson assembly, Tag added through primers P20 was Isolated from pVE4-ADRC [1]plasmid by PCR.

References

[1] V. Khasdan, “Thesis submitted in partial fulfillment : Cloning Combinations of Four Genes from Bacillus thuringiensis,” no. October, 2015.

[2] Y. Xu, M. Nagai, M. Bagdasarian, T. W. Smith, and E. D. Walker, “Expression of the p20 Gene from Bacillus thuringiensis H-14 Increases Cry11A Toxin Production and Enhances Mosquito-Larvicidal Activity in Recombinant Gram-Negative Bacteria,” Appl. Environ. Microbiol., vol. 67, no. 7, pp. 3010–3015, Jul. 2001.