Coding
Cry4Ba + H

Part:BBa_K2938006:Design

Designed by: Assaf Vital   Group: iGEM19_BGU_Israel   (2019-09-28)


Cry4Ba + HA tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1708
    Illegal SpeI site found at 2397
    Illegal PstI site found at 212
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 96
    Illegal SpeI site found at 2397
    Illegal PstI site found at 212
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1708
    Illegal SpeI site found at 2397
    Illegal PstI site found at 212
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1708
    Illegal SpeI site found at 2397
    Illegal PstI site found at 212
    Illegal NgoMIV site found at 2520
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 502


Design Notes

HA-tag fused to N- terminal


Source

Gibson assembly. Tag was added through the primers. Cry4Ba Isolated from Bacillus thuringiensis subsp. Israelensis by PCR.

References

[1] V. Khasdan, “Thesis submitted in partial fulfillment : Cloning Combinations of Four Genes from Bacillus thuringiensis,” no. October, 2015.

[2] C. Xu, B. C. Wang, Z. Yu, and M. Sun, “Structural insights into Bacillus thuringiensis Cry, Cyt and parasporin toxins,” Toxins, vol. 6, no. 9. pp. 2732–2770, 01-Sep-2014.

[3] E. Ben-Dov, “Bacillus thuringiensis subsp. israelensis and its dipteran-specific toxins.,” Toxins (Basel)., vol. 6, no. 4, pp. 1222–43, Mar. 2014.