Device

Part:BBa_K2936017

Designed by: Weng Jiaqi   Group: iGEM19_ZJUT-China   (2019-10-10)


pFrmR-AraC

pFrmR is an engineered formaldehyde-inducible promoter, that expresses the genes when the concentration of CHOH in the environment reach a certain level. The AraC protein would binds to AraBAD promoter, inhibiting its expression.

Short description

A bidirectional switch

Description

pFrmR is an engineered formaldehyde-inducible promoter, that expresses the genes when the concentration of CHOH in the environment reach a certain level. The AraC protein would binds to AraBAD promoter, inhibiting its= expression. Those two promoter and the ara operon form a bidirectional switch determined by formaldehyde concentration.

Source

Escherichia coli.

Design consideration

There is a leak problem with the FrmR promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None