Coding

Part:BBa_K2936003:Design

Designed by: Yu Weng   Group: iGEM19_ZJUT-China   (2019-10-08)


S- glutathione hydrolase(Regulated by flhA gene)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 130
    Illegal PstI site found at 610
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 130
    Illegal PstI site found at 610
    Illegal NotI site found at 971
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 130
    Illegal BglII site found at 491
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 130
    Illegal PstI site found at 610
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 130
    Illegal PstI site found at 610
    Illegal NgoMIV site found at 79
    Illegal NgoMIV site found at 275
    Illegal NgoMIV site found at 628
    Illegal NgoMIV site found at 888
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When combined with fghA gene sequence, an RBS sequence needs to be inserted between the two ends of genes to improve the translation speed.


Source

From Parazococci denitrification.

References