DNA

Part:BBa_K2935070:Design

Designed by: Haiyong Shu   Group: iGEM19_FAFU-CHINA   (2019-10-16)


a promoter of ChR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1560
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1560
    Illegal NheI site found at 351
    Illegal NheI site found at 414
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1560
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1560
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1560
    Illegal NgoMIV site found at 764
    Illegal AgeI site found at 670
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1658


Design Notes

The promoter in front of ChR gene as the photosensitive promoter. It can be activated in blue light to promote expression.

Source

Chlamydomonas reinhardtii

References