Composite

Part:BBa_K2933250:Design

Designed by: Ruoming Sun   Group: iGEM19_TJUSLS_China   (2019-09-15)


RBS b+Linker h+His+Linker a+Sumo+Linker b+PEDO-1+T7 terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 298
    Illegal PstI site found at 934
    Illegal PstI site found at 1220
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 298
    Illegal NheI site found at 75
    Illegal NheI site found at 1269
    Illegal PstI site found at 934
    Illegal PstI site found at 1220
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 298
    Illegal BglII site found at 187
    Illegal BamHI site found at 386
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 298
    Illegal PstI site found at 934
    Illegal PstI site found at 1220
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 298
    Illegal PstI site found at 934
    Illegal PstI site found at 1220
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

NCBI.

References