Composite

Part:BBa_K2933219:Design

Designed by: Pengqian Li   Group: iGEM19_TJUSLS_China   (2019-09-15)


T7 promoter+RBS b+Linker h+His+Linker f+ARL-1+T7 terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
    Illegal PstI site found at 631
    Illegal PstI site found at 745
    Illegal PstI site found at 796
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 169
    Illegal PstI site found at 631
    Illegal PstI site found at 745
    Illegal PstI site found at 796
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
    Illegal PstI site found at 631
    Illegal PstI site found at 745
    Illegal PstI site found at 796
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
    Illegal PstI site found at 631
    Illegal PstI site found at 745
    Illegal PstI site found at 796
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Urgent need to upload


Source

NCBI

References