Coding

Part:BBa_K2933117:Design

Designed by: Xueqing Fu   Group: iGEM19_TJUSLS_China   (2019-09-14)


GST+Linker+VIM-66


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1436
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


Design Notes

VIM-66 is codon optimized for BL21(DE3) E. coli.


Source

NCBI.

References