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Coding

Part:BBa_K2933110:Design

Designed by: Xueqing Fu   Group: iGEM19_TJUSLS_China   (2019-09-14)


GST+Linker+CPS-1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1235
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1235
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1517
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1235
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1235
    Illegal AgeI site found at 1136
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


Design Notes

CPS-1 is codon optimized for BL21(DE3) E. coli.

Source

NCBI.

References