Coding
Part:BBa_K2933012:Design
Designed by: Yongjie Li Group: iGEM19_TJUSLS_China (2019-09-08)
subclass B1 metallo-beta-lactamase JOHN-1, codon optimized in E. coli
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 76
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 76
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 76
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 76
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 600
Design Notes
This part is codon optimized in E. coli
Source
Flavobacterium johnsoniae