Coding

Part:BBa_K2933011:Design

Designed by: Ruihan Dong   Group: iGEM19_TJUSLS_China   (2019-09-06)


subclass B1 metallo-beta-lactamase GIM-2, codon optimized in E. coli


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 731
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 731
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 731
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 731
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Its sequence has been optimized for E.coli.


Source

From genomic sequence of Enterobacter cloacae.

References